|
KMID : 0357920010350060461
|
|
Korean Journal of Pathology
2001 Volume.35 No. 6 p.461 ~ p.469
|
|
|
Diagnostic Utility of Polymerase Chain Reaction-Based Clonality Analysis for Immunoglobulin Heavy Chain Gene and T-cell Receptor Gamma Chain Gene Rearrangement in Lymphoid Neoplasms
|
|
Cho Eun-Yoon
Ko Young-Hyeh
Kim Dae-Shick
Han Jae-Joon
Ree Howe-J.
|
|
|
Abstract
|
|
|
Background: The clonality of lymphoid infiltrates determined by polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) or T cell receptor (TCR) genes is not only useful in confirming the diagnosis of malignant lymphoma but also in establishing the lineage of a clonal lymphoid proliferation. We analyzed the efficiency of PCR analyses for IgH and TCRγgenes that have been routinely applied for the diagnosis of malignant lymphoma in our laboratory.
Methods: Paraffin sections of 200 cases were analyzed by seminested PCR. Primers were FRIIIA-LJH/VLJH consensus primer for IgH gene and Vγ-Jγ consensus primer for TCRγ gene. The cases showing negative results by PCR for TCRγ gene were further analyzed by multiplex Vγ family primers with heteroduplex analysis.
Results: PCR approach for IgH gene allowed detection of clonality in 100% of cases with false positive rate of 0.3% and false negative rate of 0%. The combination of PCR for TCRγ consensus primers with multiplex Vγ family primers allowed detection of clonality in 91% of cases with false positive rate of 0.6% and false negative rate of 10.3%.
Conclusions: Combined analysis of IgH and TCR gene rearragnements by the PCR technique followed by heteroduplex analysis can be a useful diagnostic adjunct to determine the clonality of various lymphoproliferative diseases with high sensitivity. But clinical, morphological and immunophenotypical correlation should be considered to reach the final diagnosis due to a few false positive cases.
|
|
|
KEYWORD
|
|
|
Lymphoma, Polymerase Chain Reaction
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|